rabbit anti-mouse abca1 ab  (Novus Biologicals)

Journal: JCI Insight

Article Title: Dicarbonyl-modified lipoproteins contribute to proteinuric kidney injury

doi: 10.1172/jci.insight.161878

Figure Lengend Snippet: ( A ) Proximal tubular cells (megalin-positive, green) of NEP25 proteinuric kidneys absorbed apoAI (red, top panel). Uptake of apoAI (red) also occurred independently of megalin, illustrated in NEP25/megalin KO (middle panel) and NEP25/megalin mosaic KO mice (bottom panel). ( B ) IHC staining showed greater expression of lipoprotein transporters ABCA1 and SRBI, but not ABCG1, in NEP25 mice versus WT. Scale bar: 50 μm.

Article Snippet: Sections with primary Abs were incubated overnight at 4°C by adding rabbit anti-mouse ABCA1 Ab (1:200; Novus Biologicals), rabbit anti-mouse SRBI Ab (1:200; Novus Biologicals), and rabbit anti-mouse ABCG1 Ab (1:200; Novus Biologicals).

Techniques: Immunohistochemistry, Expressing

Journal: JCI Insight

Article Title: Dicarbonyl-modified lipoproteins contribute to proteinuric kidney injury

doi: 10.1172/jci.insight.161878

Figure Lengend Snippet: ( A ) Cultured TECs exposed to IsoLG-apoAI showed higher expression of ABCA1 and SRBI versus apoAI. ( B ) TECs took up more IsoLG-apoAI versus apoAI. Scale bar: 50 μm. Knockdown of either ABCA1 or SRBI ( C and D ) reduced TEC uptake of apoAI but not IsoLG-apoAI. ( E ) In TECs exposed to apoAI, knockdown of ABCA1 or SRBI decreased expression of the other transporter. ( F ) In TECs exposed to IsoLG-apoAI, ABCA1 knockdown significantly increased SRBI expression. SRBI siRNA significantly increased ABCA1 expression. ( G ) Knockdown of both ABCA1 and SRBI reduced cellular uptake IsoLG-apoAI. In vitro, experiments were performed independently 3 times with 3 wells per treatment. Data represent mean ± SEM. Overall statistical difference determined by Kruskal-Wallis test and pairwise difference by Wilcoxon rank sum test followed by Bonferroni correction on P values. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Sections with primary Abs were incubated overnight at 4°C by adding rabbit anti-mouse ABCA1 Ab (1:200; Novus Biologicals), rabbit anti-mouse SRBI Ab (1:200; Novus Biologicals), and rabbit anti-mouse ABCG1 Ab (1:200; Novus Biologicals).

Techniques: Cell Culture, Expressing, Knockdown, In Vitro